畢業(yè)論文 甲醛脅迫對洋蔥內(nèi)表皮細胞凋亡的誘導.doc
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畢業(yè)論文 甲醛脅迫對洋蔥內(nèi)表皮細胞凋亡的誘導,目錄1.論文題目、姓名、學院........................................... 22.中文摘要、英文摘要...........................................2-33. 引言..........................................
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目錄
1.論文題目、姓名、學院........................................... 2
2. 中文摘要、英文摘要...........................................2-3
3. 引言..........................................................3
4.實驗材料與方法步驟:.............................................3
4.1實驗材料..................................................................................... 3
4.2方法步驟：.................................................3
4.2.1材料處理...............................................3
4.2.1.1預處理...........................................3
4.2.1.2取材.............................................4
4.2.1.3處理.............................................4
4.2堿性品紅溶液染色及形態(tài)學觀測................................4
4.3細胞凋亡計數(shù)統(tǒng)計............................................4
5.結果與分析......................................................4
5.1細胞凋亡的形態(tài)學變化.....................................4-5
5.2細胞凋亡率................................................5
6.討論..........................................................5-6
7.參考文獻......................................................6-7
8.致謝...........................................................7
甲醛脅迫對洋蔥內(nèi)表皮細胞調(diào)往的誘導
付濤，生命科學學院
摘要： 細胞凋亡是當今環(huán)境毒理學領域的研究熱點之一．為了探討甲醛能否誘導細胞凋亡，以洋蔥內(nèi)表皮細胞為實驗材料，通過不同濃度的甲醛處理一段時間后，應用形態(tài)學觀察、顯微鏡觀察實驗檢測了洋蔥內(nèi)表皮細胞凋亡情況．實驗結果表明低濃度甲醛 (≤0．2mmol•L)處理洋蔥內(nèi)表皮細胞一段時間后，對細胞生長有顯著的抑制作用 ，但對洋蔥內(nèi)表皮細胞形態(tài)改變不大，且對細胞核DNA沒有損傷；而高濃度甲醛 (≥0．4mmol•L)處理細胞 一段時間后，對細胞生長有極顯著的抑制作用，細胞形態(tài)逐漸變的模糊不清，并有少量細胞萎縮成圓形， 且會造成細胞核DNA有規(guī)律的斷裂，細胞凋亡率隨甲醛濃度的增加而增大．以上實驗結果顯示，高濃度甲醛處理洋蔥內(nèi)表皮細胞一段時間后，會誘導細胞凋亡；并且隨甲醛濃度的升高凋亡率逐漸 增大，呈一定的劑量一效應關系．
關鍵詞：細胞凋亡；甲醛；洋蔥內(nèi)表皮細胞；顯微鏡
Abstract： Nowadays apoptosis has gained much interest in the environmentaltoxicology．In order to explore whether formaldehyde could induce apoptosis or not， Onion epidermal cells were treated with different concentrations of fomraldehyde for a period of time ．Morphologic observation、Microscopic observation of experimental were applied to detect the apoptosis of Onion epidermal cells in this experiment．Results showed that Onion epidermal cells’ prolifeation was significantly inhibited alter treated with FA atlow concentrations (≤ 0．2mmol/L )ina period of time ，while morphological change of cells was negligible，and no significant damage of cellular DNA wasobserved under the conditions．However，Onion epidermal cells’ proliferation was statistically significantly inhibited after treated with FA a thigh concentrations(≥0．4mmol/L)ina period of time ，nada small amount of cells shrank intorotundity．Meanwhile，cell nucleus DNA was broken intoregularfragment，and apoptosis rate was increased with the elevation of FA concentrations．These experiments showed hat the treatment of Onion epidermalcells by FA a thigh concentrations of r a period of time might induce cell apoptosis；andtherewasna obviously dose—depend entre lationship between FA concentrations and apoptosisrate．
Key words：apoptosis； formaldehyde；Onion epidermal cells；microscope
1.論文題目、姓名、學院........................................... 2
2. 中文摘要、英文摘要...........................................2-3
3. 引言..........................................................3
4.實驗材料與方法步驟:.............................................3
4.1實驗材料..................................................................................... 3
4.2方法步驟：.................................................3
4.2.1材料處理...............................................3
4.2.1.1預處理...........................................3
4.2.1.2取材.............................................4
4.2.1.3處理.............................................4
4.2堿性品紅溶液染色及形態(tài)學觀測................................4
4.3細胞凋亡計數(shù)統(tǒng)計............................................4
5.結果與分析......................................................4
5.1細胞凋亡的形態(tài)學變化.....................................4-5
5.2細胞凋亡率................................................5
6.討論..........................................................5-6
7.參考文獻......................................................6-7
8.致謝...........................................................7
甲醛脅迫對洋蔥內(nèi)表皮細胞調(diào)往的誘導
付濤，生命科學學院
摘要： 細胞凋亡是當今環(huán)境毒理學領域的研究熱點之一．為了探討甲醛能否誘導細胞凋亡，以洋蔥內(nèi)表皮細胞為實驗材料，通過不同濃度的甲醛處理一段時間后，應用形態(tài)學觀察、顯微鏡觀察實驗檢測了洋蔥內(nèi)表皮細胞凋亡情況．實驗結果表明低濃度甲醛 (≤0．2mmol•L)處理洋蔥內(nèi)表皮細胞一段時間后，對細胞生長有顯著的抑制作用 ，但對洋蔥內(nèi)表皮細胞形態(tài)改變不大，且對細胞核DNA沒有損傷；而高濃度甲醛 (≥0．4mmol•L)處理細胞 一段時間后，對細胞生長有極顯著的抑制作用，細胞形態(tài)逐漸變的模糊不清，并有少量細胞萎縮成圓形， 且會造成細胞核DNA有規(guī)律的斷裂，細胞凋亡率隨甲醛濃度的增加而增大．以上實驗結果顯示，高濃度甲醛處理洋蔥內(nèi)表皮細胞一段時間后，會誘導細胞凋亡；并且隨甲醛濃度的升高凋亡率逐漸 增大，呈一定的劑量一效應關系．
關鍵詞：細胞凋亡；甲醛；洋蔥內(nèi)表皮細胞；顯微鏡
Abstract： Nowadays apoptosis has gained much interest in the environmentaltoxicology．In order to explore whether formaldehyde could induce apoptosis or not， Onion epidermal cells were treated with different concentrations of fomraldehyde for a period of time ．Morphologic observation、Microscopic observation of experimental were applied to detect the apoptosis of Onion epidermal cells in this experiment．Results showed that Onion epidermal cells’ prolifeation was significantly inhibited alter treated with FA atlow concentrations (≤ 0．2mmol/L )ina period of time ，while morphological change of cells was negligible，and no significant damage of cellular DNA wasobserved under the conditions．However，Onion epidermal cells’ proliferation was statistically significantly inhibited after treated with FA a thigh concentrations(≥0．4mmol/L)ina period of time ，nada small amount of cells shrank intorotundity．Meanwhile，cell nucleus DNA was broken intoregularfragment，and apoptosis rate was increased with the elevation of FA concentrations．These experiments showed hat the treatment of Onion epidermalcells by FA a thigh concentrations of r a period of time might induce cell apoptosis；andtherewasna obviously dose—depend entre lationship between FA concentrations and apoptosisrate．
Key words：apoptosis； formaldehyde；Onion epidermal cells；microscope
