葛根總黃酮的提取分離方法研究

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[摘要] 目的 建立一套有效的提取及初步分離葛根中葛根素的方法。方法 使用紫外分光光度法、薄層色譜及高效液相色譜對(duì)葛根素純品、大豆甙元純品和葛根的初純化物進(jìn)行比較鑒定。結(jié)果 采用回流法提取、萃取法純化是一種產(chǎn)量較大、純度很高而且簡(jiǎn)單易行的方法，較適用于工業(yè)化大生產(chǎn)。結(jié)論 回流法提取，萃取法純化是一種較合適的方法。

[關(guān)鍵詞] 葛根，黃酮，提取分離

Abstract: Objective In order to set up a set of effective Extraction and separation of Flavonoids in Radix Puerariae. Methods Reflux and Extraction were adopted. And the Flavonoids in Radix Puerariae were determined by UV spectrophotometer, Thin Layer Chromatography and High Performance Liquid Chromatography. Results Reflux is a good method of Extraction and Extraction is a effective method of Separation. Adopting them, we can get high quality extraction. They are suitable for industrial production. Conclusion It indicates that Reflux and Extraction are effective method of Extraction and Separation of Flavonoids in Radix Puerariae
Key words: Radix Pueraria; flavonoid; extraction and separation
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